FastQCFastQC Report
Wed 5 Dec 2018
read_2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenameread_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length21-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55092.2036No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27571.1028No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.6624No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.6335999999999999No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.5668000000000001No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.46759999999999996No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.3896No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.3152No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.1812No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3380.1352No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2720.1088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT207.471848E-444.4371531
TGGGGGG3600.042.5856061
CTCGAGT401.6956563E-533.327861
AGGGGGG450.00131702524.6873071
ATTTCGG500.002433937322.21412710
CCCCCCC24850.021.3691731
ATCTACA550.004229922320.19466218
AGGCAGC550.004229922320.19466210
TCCAGGA800.001858106716.6605977
TCAACCA953.09588E-416.3683057
CCCCCCT13400.015.0857143
CTCCTTT1759.3301605E-713.9659611
CCCCCTT15300.013.3575154
CCCCTTT16850.012.1287825
GGGGAAA2707.3298E-811.52304929
CTCCATT1350.00438553411.5207421
CCCTTTT17950.011.3855146
CCTTTTT18900.010.8132277
GGGGGAA2751.1580396E-610.50332628
GGGGGGA3003.1485797E-710.36866927
Produced by FastQC (version 0.11.5)