Basic Statistics
Measure | Value |
---|---|
Filename | read_2.fq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 250000 |
Sequences flagged as poor quality | 0 |
Sequence length | 35-51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 5509 | 2.2036 | No Hit |
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2757 | 1.1028 | No Hit |
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1656 | 0.6624 | No Hit |
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1584 | 0.6335999999999999 | No Hit |
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1417 | 0.5668000000000001 | No Hit |
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1169 | 0.46759999999999996 | No Hit |
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 974 | 0.3896 | No Hit |
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 788 | 0.3152 | No Hit |
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 453 | 0.1812 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 338 | 0.1352 | No Hit |
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN | 272 | 0.1088 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CCGGTAT | 20 | 7.394916E-4 | 44.530624 | 1 |
TGGGGGG | 360 | 0.0 | 42.675182 | 1 |
CTCGAGT | 40 | 1.6714475E-5 | 33.39797 | 1 |
AGGGGGG | 45 | 0.0013009962 | 24.739237 | 1 |
ATTTCGG | 50 | 0.0024044104 | 22.260859 | 10 |
CCCCCCC | 2485 | 0.0 | 21.414124 | 1 |
ATCTACA | 55 | 0.0041787773 | 20.237143 | 18 |
AGGCAGC | 55 | 0.0041787773 | 20.237143 | 10 |
TCCAGGA | 80 | 0.0018321791 | 16.695642 | 7 |
CCCCCCT | 1340 | 0.0 | 15.117447 | 3 |
CTCCTTT | 175 | 9.111627E-7 | 13.995339 | 1 |
CCCCCTT | 1530 | 0.0 | 13.385613 | 4 |
TCAAGTT | 135 | 2.8886713E-4 | 13.191619 | 8 |
CCCCTTT | 1685 | 0.0 | 12.154296 | 5 |
GGGGAAA | 270 | 7.1193426E-8 | 11.547288 | 29 |
CTCCATT | 135 | 0.0043172785 | 11.544976 | 1 |
CCCTTTT | 1795 | 0.0 | 11.409464 | 6 |
CCTTTTT | 1890 | 0.0 | 10.835973 | 7 |
GGGGGAA | 275 | 1.1271768E-6 | 10.52542 | 28 |
GGGGGGA | 300 | 3.05894E-7 | 10.390478 | 27 |