FastQCFastQC Report
Tue 1 May 2018
read_2.fq

Summary

[OK]Basic Statistics

MeasureValue
Filenameread_2.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length35-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55092.2036No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27571.1028No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.6624No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15840.6335999999999999No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14170.5668000000000001No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.46759999999999996No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.3896No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.3152No Hit
CCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.1812No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3380.1352No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2720.1088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT207.394916E-444.5306241
TGGGGGG3600.042.6751821
CTCGAGT401.6714475E-533.397971
AGGGGGG450.001300996224.7392371
ATTTCGG500.002404410422.26085910
CCCCCCC24850.021.4141241
ATCTACA550.004178777320.23714318
AGGCAGC550.004178777320.23714310
TCCAGGA800.001832179116.6956427
CCCCCCT13400.015.1174473
CTCCTTT1759.111627E-713.9953391
CCCCCTT15300.013.3856134
TCAAGTT1352.8886713E-413.1916198
CCCCTTT16850.012.1542965
GGGGAAA2707.1193426E-811.54728829
CTCCATT1350.004317278511.5449761
CCCTTTT17950.011.4094646
CCTTTTT18900.010.8359737
GGGGGAA2751.1271768E-610.5254228
GGGGGGA3003.05894E-710.39047827
Produced by FastQC (version 0.11.5)